We routinely perform the following procedures in our laboratory:
| Molecular Biology | - PCR methods and gel electrophoresis of DNA
- Cloning methods for plasmids for protein expression
|
| Mikrobiology | - Possibilities of screening microorganisms or enzymes on a microtiter scale
- Microbial growth tests in shake flasks
- Optimization of fermentation parameters on a 1L scale
- Adaptive Laboratory Evolution (e.g. to adapt microorganisms for sidestream utilization)
|
| Protein Biochemistry | - Protein analysis using SDS-PAGE
- Western blotting
- Enzyme/protein purification via affinity-tags
- Enzyme activity tests (luminescence, absorbance)
|
| Genetic Engineering | - Transformation of E. coli
- Modifications of A. niger mittels using established CRISPR/Cas9-technology
|
In cooperation with the laboratories for microbiology and molecular biology we work with the following equipment:
| Cultivation | - 2 coolable and heatable incubation shakers | Innova?42 from New Brunswick / Eppendorf
- several incubation chambers | IFE500 von Memmert, TIN-IN35 PLUS from Phoenix Instruments
- 2 benchtop fermenters for 1L working volume | MINIFORS2 from Infors HT
|
| Sample preparation | - several, coolable centrifuges for 1 ml to 250 ml sample volumes | 5804R von Eppendorf, CD-3124R from Phoenix Instruments
- sterile workbench | HERASAFE KS 18 from Thermo
- several automatic pipette sets including mulit-channel pipettes | 1 ?l to 1 ml from Eppendorf
|
| Evaluation | - microtiter plate reader | SPARK? with te-inject from Tecan
- cuvette photometer | Libra S12 from biochrom
- gel documentation system | GelDoc XR+ from Bio-Rad
- Cell Counter | TC20 from Bio-Rad
- PCR-Cycler | Gene Explorer 48/48 Gradient from Biozym
- various thermal shakers | for 1-2 ml sample vessels from Eppendorf and CellMedia
- Moisture analyzer | MBG163L from VWR
- gel electrophoresis chambers for agarose gels
- SDS-PAGE equipment | Mini-PROTEAN Tetra Vertical Electrophoresis Cell from Bio-Rad
- semi-dry-protein blotter | Trans-Blot Turbo Transfer System from Bio-Rad
- Sonotrode | Sonoplus HD 4100 from Bandelin
|
| Storage & Disposal | - ultra-low temperature freeezer | CryoCube F101h from Eppendorf
- several autoclaves | VK-95 and DX-45 from Systec
|